In vitro PLANTLET REGENERATION APTITUDE OF SIX MANDARINS VARIETIES FROM CULTURED LEAF SEGMENT (Citrus reticulata)

Main Article Content

LABEL KAWTAR
HANDAJI NAJAT
BRHADDA NAJIBA
GMIRA NAJIB
ZIRI RABEA
RAJAA YACOUBI
BENYAHIA HAMID

Abstract

Developing programs for genetic citrus improvement with plant biotechnology enables to use tools of precocious in vitro selection and somatic hybridization, making new opportunities in exploring citrus germplasm variability. This study aims at evaluating the effect of hormonal composition of the medium on friable callus induction, and regeneration of plants by organogenesis from calluses. Leaf segment of six varieties of mandarins was cultured on MT medium supplemented with growth regulators (2,4-dichlorophenoxyacetic acid. -naphthaleneacetic acid (NAA)] and cytokinin [6-benzyl amino purine (BAP)] were investigated in order to determine the suitable callus induction. The four varieties; namely, Murcott, Temple, Anana and Ortanique demonstrated a higher callus induction capacity. The combinations 2.4-D alone or combined with the BAP are the most reactive which led 60% of explants to form callus. These same culture media showed a gradual increase in the percentages of root appearance or caulinary neoformations. The shoots were rooted in the medium containing ANA (1 mg/l) Well-developed seedlings with healthy roots were successfully acclimated. Developing a callus induction and regeneration protocol would lead to mass propagation and in vitro production of seedlings with somaclonal variations.

Keywords:
Citrus, callogenesis, in vitro regeneration, mandarins, organogenesis.

Article Details

How to Cite
KAWTAR, L., NAJAT, H., NAJIBA, B., NAJIB, G., RABEA, Z., YACOUBI, R., & HAMID, B. (2019). In vitro PLANTLET REGENERATION APTITUDE OF SIX MANDARINS VARIETIES FROM CULTURED LEAF SEGMENT (Citrus reticulata). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 20(21-22), 937-950. Retrieved from https://www.ikpresse.com/index.php/PCBMB/article/view/4769
Section
Original Research Article

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Jajoo A. In vitro propagation of Citrus limonia Osbeck. through nucellar embryo culture. Current Research Journal of Biological Sciences. 2010;2(1):6-8.

Lombardo G, Alessandro R, Scialabba A, Sciandra M. Direct organogenesis from cotyledons in cultivars of Citrus clementina Hort. Ex Tan. American Journal of Plant Sciences. 2011;2(2):237-244.

Handaji N, Larsalone N, Inmarti At, Dambier D, Benyahia H, Maiguizo H, Cheikh OY, Ollitrault P. Induction de l’embryogenèse somatique et régénartion des plantules chez les mandariniers (Citrus reticulata L.). AL AWAMIAI. 2005;2(2).

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Label K, Handaji N, Brhadda N, Gmira N, Ziri R, Mahmoudi K, Essalhi E , Ennaciri H, Hmimidi A, Benyahia H. Histological study of somatic embryogenesis of mandarins(Citrus réticulata). Plant Cell Biotechnology and Molecular Biology. 2019;15-16:617–633.

Khaleda L, Al-Forkan M. Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deep water rice (Oryza sativa L.) cultivars of Bangladesh. African Journal of Biotechnology. 2006;5(16):1435-1440.

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Alka J. In vitro propagation of Citrus limonia through nucellar embryo culture. Current Research Journal of Biological Sciences. 2010;2(1):6-8.

Pe´rez-Tornero O, Tallon OC and Porras I. An efficient protocol for micropropagation of lemon (Citrus limon) from mature nodal segments. Plant Cell Tiss. Org. Cult. 2010;100:263-271.

Tallon CI, Porras I, Perez-Tornero O. High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium. In Vitro Cellular and Developmental Biology – Plant. 2013;49:145-155.

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Kumar S, Kanwar JK. Plant regeneration from cell suspensions in Gerbera jamesonii Bolus. Journal of Fruit and Ornamental Plant Research. 2007;15:157-166.

Kumar S, Kanwar JK. Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro. Folia Horticulturae. 2006;18:57-64.

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Barlass M, Skene KGM. In vitro plantlet formation from Citrus species and hybrids. Scientia Horticulturae, Amsterdam. 1982;17(4):333-341.

Moreira-Dias JM, Molina RV, Bordzxloacute NY, Guardiola JL, Garczxliacute. Direct and indirect shot organogenic pathways in epicotyls cuttings of Troyer citrange in hormone requierement and their response ti light. Ann Bot. 2000;85:103-110.

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Jimenez VM. Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant growth regulations. 2005;47:91-110.

Behar N, Kumar P, Chandel G. Effect of explant type, genotype and plant growth regulators on morphogenetic potential of flax (Linum usitatissimum L.). Journal of Cell and Plant Sciences. 2011; 2(1):13-18.

Thaniarasu R, Kumar TS, Rao M. Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants. Physiol. Mol. Biol. Plants. 2016;22:143-151.

Abdullah S, Khan FA, Iftikhar I, Raza M, Rasheda Aslam M, Ghazanfar Hammad A, Zafar W, Usman Ijaz M. Detection of somaclonal variation in micropropagated plants of sugarcane and SCMV screening through ELISA. Journal of Agricultural Science; 2013.

Ge F, Luo X, Huang X, Zhang Y, He X, Liu M. Genome-wide analysis of transcription factors involved in maize embryonic callus formation. Physiol. Plant. 2016;158:452–462.

Molina RV, Castelló S, García-Luis A and Guardiola JL. Light cytokinin interaction in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro. Plant Cell Tissue and Organ Culture. 2007;89:131-140.

Cervera M, Navarro AA, Navarro L, Peña L. Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration. Tree Physiology. 2008;28:55–66.

Esmaeilnia E, Dehestani A. In vitro plant regeneration from mature tissues of Thomson navel sweet orange (Citrus sinensis L. Osbeck.). Biharean Biologist. 2015;9:9-14.

Drone S, Jullien F, Caissard JC, Faure OA. simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula×intermedia Emeric ex Loiseleur). Plant Cell Rep. 1999;18:429–33.

Saadi A, Hamdani FZ. Régénération in vitro du Scorpiurus muricatus ssp. subvillosus via la caulogenèse. Biotechnol. Agron. Soc. Environ. 2007;11(3):185–191.

Gharnit N, Ennabili A. Essais preliminaires de culture in vitro du caroubier (ceratonia siliqua L.) Originaire du nord-ouest du maroc. Biomatec Echo. 2009;3(6):18-25.

Meziane M, Boudjeniba M, Frasheri D, D’Onghia AM, Carra A, Carimi F, Braneci S. Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis. African Journal of Biotechnology. 2012;11(25):6666.

Gill MIS, Singh Z, Dhillon BS, Gosal SS, Singh Z. Somatic embryogenesis and plantlet regeneration on calluses derived from seedling explants of kinnow mandarin (Citrus nobilis Lour × Citrus deliciosa Tenora). J. Hortic. Sci. 1994;69:231-236.

Phillips GC, Collins GB. In vitro tissue culture of delected legumes and plant regeneration from callus cultures of red clover. Crop Sci. 1979;19:59–62.

Walker KA, Wendeln ML, Jaworski EC. Organogenesis in callus tissue of Medicago sativa L. The temporal separation of induction processes from differentiation processes. Plant Sci. Lett. 1979;16:23–30.

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Brhadda N, Abousalim A, Walali LDM. Effets du milieu de culture et de la lumière sur l’embryogenèse somatique de l’olivier (Olea europaea L.) cv Picholine marocaine Fruit. 2003;85(3):1–14.

Brhadda N, Walalli LDM, Abousalim A. Rizogenèse et regeneration in vitro de plantules d’olivier (Olea europaea L.) variété picholine marocaine. 1999;1-30.

Saadi A, Hamdani F. Régénération in vitro du Scorpiurus muricatus ssp. subvillosus via la caulogenèse. Biotechnol. Agron. Soc. Environ. 2007;11(3):185–191.

Benyahia H, Chetto O, Dominique D, Froelicher Y. Mise au point des conditions de calogenèse, caulogenèse et rhizogenèse chez les porte-greffes d’agrumes à partir d’épicotyle: Cas du citrange Troyer. Journal of Applied Biosciences. 2012;60:4375–4387.

[ISSN 1997–5902]

Shawkat A, Bushramirza. Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explant type and hormone concentration. Acta Bot. Croat. 2006;65(2):137–146.

Das A, Paul AK, Chaudhuri S. Micropropagation of sweet orange (Citrus sinensis Osbeck) for the development of nucellar seedlings. Indian J. Exp. Biol. 2000;38:269–72.

Sani L and Mustapha Y. Effect of genotype and 2, 4- d concentration on callogenesis in sugarcane (Saccharum spp. hybrids). Bayero Journal of Pure and Applied Sciences. 2010;3(1):238–240.

Khan MS, Zaidi A, Wani PA, Ove M. Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils. Environ. Chem. Lett. 2009;7:1–19.

Chaturvedi HC, Mitra GC. Clonal propagation of Citrus from somatic callus cultures. Horticultue Science. 1974;9:118-120.

Beloualy N. Plant regeneration from callus culture of three citrus rootstocks. Plant Cell Tissue and Organ Culture. 1991;24:29-34.

Savita V, Virk GS, Avinash Nagpal. Effect of explant type and different plant growth regulators on callus induction and plantlet regeneration in Citrus jambhiri Lush. Environment & We An International Journal of Science & Technology. 2010;5:97-106.

Moreira-Dias JM, Molina RV, Guardiola JL, Garcia-louis A. Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange. Scientia Horticulturae, Amster¬dam. 2001;87:275-290.

Almeida WAB, Mourao Filho FAA, Mendes BMJ, Rodriguez APM. In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia. Sci Agr. 2002;59:35-40.

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Jajoo A. In vitro propagation of Citrus limonia Osbeck. through nucellar embryo culture. Current Research Journal of Biological Sciences. 2010;2(1):6-8.

Lombardo G, Alessandro R, Scialabba A, Sciandra M. Direct organogenesis from cotyledons in cultivars of Citrus clementina Hort. Ex Tan. American Journal of Plant Sciences. 2011;2(2):237-244.

Handaji N, Larsalone N, Inmarti At, Dambier D, Benyahia H, Maiguizo H, Cheikh OY, Ollitrault P. Induction de l’embryogenèse somatique et régénartion des plantules chez les mandariniers (Citrus reticulata L.). AL AWAMIAI. 2005;2(2).

Label K, Handaji N, Brhadda N, Elsalhi E, benyahia H. Optimisation de l’induction de callogenèse à partir des embryons excisés de mandariniers (Citrus sp.). Journal of Applied Biosciences. 2015;89(1):8300-8310.

Label K, Handaji N, Brhadda N, Gmira N, Ziri R, Mahmoudi K, Essalhi E , Ennaciri H, Hmimidi A, Benyahia H. Histological study of somatic embryogenesis of mandarins(Citrus réticulata). Plant Cell Biotechnology and Molecular Biology. 2019;15-16:617–633.

Khaleda L, Al-Forkan M. Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deep water rice (Oryza sativa L.) cultivars of Bangladesh. African Journal of Biotechnology. 2006;5(16):1435-1440.

Altaf N, Khan AR. Growth and development of low seeded Kinnow mandarin fruit in dense plantation. J Agric Sci Technol. 2009;11:191-198.

Alka J. In vitro propagation of Citrus limonia through nucellar embryo culture. Current Research Journal of Biological Sciences. 2010;2(1):6-8.

Pe´rez-Tornero O, Tallon OC and Porras I. An efficient protocol for micropropagation of lemon (Citrus limon) from mature nodal segments. Plant Cell Tiss. Org. Cult. 2010;100:263-271.

Tallon CI, Porras I, Perez-Tornero O. High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium. In Vitro Cellular and Developmental Biology – Plant. 2013;49:145-155.

Huang T, Peng S, Dong G, Zhang Li. Plant regeneration from leaf derived callus in Citrus grandis (Pummelo): Effects of auxin in callus induction medium. Plant Cell Tiss. Org. Cult. 2002;69:141–146.

Almeida WAB, Mourao Filho FAA, Mendes BMJ, Rodriguez APM. In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia. Scientia Agricola. Piracicaba. 2002;59(1):35-40.

Hao YJ, Wen XP, Deng XX. Genetic and epigenetic evaluations of Citrus calluses recovered from slow-growth culture. J Plant Physiol. 2004;161:479–484.

Grosser JW, Gmitter FG, Jr, Dutt M, Calovic M, Ling P, Castle B. Highlights of the University of Florida, Citrus Research and Education Center’s comprehensive citrus breeding and genetics program. Acta Hortic. 2015;1065:405–413.

Siragusa M, Carra A, Salvia L, Puglia A, De Pasquale F, Carimi F. Genetic instability in calamondin (Citrus madurensis Lour.) plants derived from somatic embryogenesis induced by diphenylurea derivatives. Plant Cell Rep. 2007;26:1289–1296.

Hao YJ. In vitro conservation and genetic variation of important fruit trees. PhD thesis. Huazhong Agricultural University. Wuhan, P. R. China; 2000.

Ding L, Li S, Gao J, Wang Y, Yang G, He G. Optimization of agrobacterium-mediated transformation conditions in mature embryos of elite wheat. Mol Biol. 2009;36:29-36.

Anil V, Spurti Bennur, Savitha Lobo. Somaclonal variations for crop improvement: Selection for disease resistant variants in vitro Plant Science Today. 2018;5(2):44-54.

Nwauzoma, Jaja. A review of somaclonal variation in plantain a review of somaclonal variation in plantain (Musa spp): Mechanisms and applications. Journal of Applied Biosciences. 2013;67:5252– 5260.

Murashige T, Tucker DPH. Growth factor requirements of Citrus tissue culture. Proc. First Intern. Citrus Symp. 1969;3:1155–1161.

Kumar S, Kanwar JK. Plant regeneration from cell suspensions in Gerbera jamesonii Bolus. Journal of Fruit and Ornamental Plant Research. 2007;15:157-166.

Kumar S, Kanwar JK. Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro. Folia Horticulturae. 2006;18:57-64.

Peters A. Quelques aspects de la morphogenèse des ignames cultivées in vitro. Mémoire de fin d’études de l’Institut Supérieur des Techniques d’Outre-mer (ISTOM), 74ème Promotion. 1986;61.

Barlass M, Skene KGM. In vitro plantlet formation from Citrus species and hybrids. Scientia Horticulturae, Amsterdam. 1982;17(4):333-341.

Moreira-Dias JM, Molina RV, Bordzxloacute NY, Guardiola JL, Garczxliacute. Direct and indirect shot organogenic pathways in epicotyls cuttings of Troyer citrange in hormone requierement and their response ti light. Ann Bot. 2000;85:103-110.

Costa MGC, Alves VS, Lani ERG, Mosquima PR, Carvalho CR, Otoni WC. Morphogenic gradients of adventitious bud and shoot regeneration in epicotyl explants of Cit¬rus. Scientia Horticulturae, Amsterdam. 2004;100:63-74. Crop Improvement. 2004;32:465.

Jimenez VM. Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant growth regulations. 2005;47:91-110.

Behar N, Kumar P, Chandel G. Effect of explant type, genotype and plant growth regulators on morphogenetic potential of flax (Linum usitatissimum L.). Journal of Cell and Plant Sciences. 2011; 2(1):13-18.

Thaniarasu R, Kumar TS, Rao M. Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants. Physiol. Mol. Biol. Plants. 2016;22:143-151.

Abdullah S, Khan FA, Iftikhar I, Raza M, Rasheda Aslam M, Ghazanfar Hammad A, Zafar W, Usman Ijaz M. Detection of somaclonal variation in micropropagated plants of sugarcane and SCMV screening through ELISA. Journal of Agricultural Science; 2013.

Ge F, Luo X, Huang X, Zhang Y, He X, Liu M. Genome-wide analysis of transcription factors involved in maize embryonic callus formation. Physiol. Plant. 2016;158:452–462.

Molina RV, Castelló S, García-Luis A and Guardiola JL. Light cytokinin interaction in shoot formation in epicotyl cuttings of Troyer citrange cultured in vitro. Plant Cell Tissue and Organ Culture. 2007;89:131-140.

Cervera M, Navarro AA, Navarro L, Peña L. Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration. Tree Physiology. 2008;28:55–66.

Esmaeilnia E, Dehestani A. In vitro plant regeneration from mature tissues of Thomson navel sweet orange (Citrus sinensis L. Osbeck.). Biharean Biologist. 2015;9:9-14.

Drone S, Jullien F, Caissard JC, Faure OA. simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula×intermedia Emeric ex Loiseleur). Plant Cell Rep. 1999;18:429–33.

Saadi A, Hamdani FZ. Régénération in vitro du Scorpiurus muricatus ssp. subvillosus via la caulogenèse. Biotechnol. Agron. Soc. Environ. 2007;11(3):185–191.

Gharnit N, Ennabili A. Essais preliminaires de culture in vitro du caroubier (ceratonia siliqua L.) Originaire du nord-ouest du maroc. Biomatec Echo. 2009;3(6):18-25.

Meziane M, Boudjeniba M, Frasheri D, D’Onghia AM, Carra A, Carimi F, Braneci S. Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis. African Journal of Biotechnology. 2012;11(25):6666.

Gill MIS, Singh Z, Dhillon BS, Gosal SS, Singh Z. Somatic embryogenesis and plantlet regeneration on calluses derived from seedling explants of kinnow mandarin (Citrus nobilis Lour × Citrus deliciosa Tenora). J. Hortic. Sci. 1994;69:231-236.

Phillips GC, Collins GB. In vitro tissue culture of delected legumes and plant regeneration from callus cultures of red clover. Crop Sci. 1979;19:59–62.

Walker KA, Wendeln ML, Jaworski EC. Organogenesis in callus tissue of Medicago sativa L. The temporal separation of induction processes from differentiation processes. Plant Sci. Lett. 1979;16:23–30.

Brhadda N, Walali LDM, Abousalim A. Etude de l'effet du type et de la concentration de cytokinines sur la prolifération de l'olivier (Olea europaea L.) cv. ' Picholine marocaine '. In : Actes des 8è Journées scientifiques. AUF, Marrakech. 2002;118-120.

Brhadda N, Abousalim A, Walali LDM. Effets du milieu de culture et de la lumière sur l’embryogenèse somatique de l’olivier (Olea europaea L.) cv Picholine marocaine Fruit. 2003;85(3):1–14.

Brhadda N, Walalli LDM, Abousalim A. Rizogenèse et regeneration in vitro de plantules d’olivier (Olea europaea L.) variété picholine marocaine. 1999;1-30.

Saadi A, Hamdani F. Régénération in vitro du Scorpiurus muricatus ssp. subvillosus via la caulogenèse. Biotechnol. Agron. Soc. Environ. 2007;11(3):185–191.

Benyahia H, Chetto O, Dominique D, Froelicher Y. Mise au point des conditions de calogenèse, caulogenèse et rhizogenèse chez les porte-greffes d’agrumes à partir d’épicotyle: Cas du citrange Troyer. Journal of Applied Biosciences. 2012;60:4375–4387.

[ISSN 1997–5902]

Shawkat A, Bushramirza. Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explant type and hormone concentration. Acta Bot. Croat. 2006;65(2):137–146.

Das A, Paul AK, Chaudhuri S. Micropropagation of sweet orange (Citrus sinensis Osbeck) for the development of nucellar seedlings. Indian J. Exp. Biol. 2000;38:269–72.

Sani L and Mustapha Y. Effect of genotype and 2, 4- d concentration on callogenesis in sugarcane (Saccharum spp. hybrids). Bayero Journal of Pure and Applied Sciences. 2010;3(1):238–240.

Khan MS, Zaidi A, Wani PA, Ove M. Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils. Environ. Chem. Lett. 2009;7:1–19.

Chaturvedi HC, Mitra GC. Clonal propagation of Citrus from somatic callus cultures. Horticultue Science. 1974;9:118-120.

Beloualy N. Plant regeneration from callus culture of three citrus rootstocks. Plant Cell Tissue and Organ Culture. 1991;24:29-34.

Savita V, Virk GS, Avinash Nagpal. Effect of explant type and different plant growth regulators on callus induction and plantlet regeneration in Citrus jambhiri Lush. Environment & We An International Journal of Science & Technology. 2010;5:97-106.

Moreira-Dias JM, Molina RV, Guardiola JL, Garcia-louis A. Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange. Scientia Horticulturae, Amster¬dam. 2001;87:275-290.

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