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Rice is a staple crop grown in the world, providing food for more than half of the population. Hybrid rice is a useful method for increasing rice yield. Many diseases and insects attack the rice plant. Among the insect pests, planthoppers cause significant yield losses. Brown planthopper (BPH) is a typical vascular feeder and one of the most serious and destructive rice pests that can be found throughout the rice-growing area. It causes severe yield reduction by directly sucking the plant sap and acting as a vector of various viral diseases such as rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV). Heavy infestations cause complete drying and plant death, a condition is known as hopper burn. The deployment of host resistance has been recognized as one of the most economical and effective methods for Brown planthopper (BPH) management. Many donors of Brown planthopper (BPH) resistance have been identified and genetic characterization of the resistance has been investigated. To date, more than 38 major Brown planthoppers (BPH) resistance genes have been reported in several Oryza sativa sps. indica cultivars and wild relatives. Two near-isogenic lines (NILs) with introgression Bph3 from Vellathu cheera (IRGC54827) trough marker-assisted breeding (MAB) and Bioassays method. RM589 SSR (simple sequence repeats) marker is linked to the Bph3 resistance gene, which is used for genotype screening the populations. Biotypes 4 insects were used for phenotypic screening. Phenotype screening showed that the improved near-isogenic lines (NILs) and their derived hybrids carrying Bph3 gene were resistant to Brown planthopper (BPH), the improved hybrids showed superior agronomic performance. Vellathu cheera donor line contains Bph3 gene, it gives more resistance for Brown planthopper (BPH) this study demonstrates that it is essential to Introgres the BPH3 gene into hybrid parental lines for developing Brown planthopper (BPH) resistant hybrid.
Heinrichs EA, Medrano FG, Rapusas HR. Genetic evaluation for insect resistance in rice. IRRI, Los Banos, Philip- pines; 1985.
IRRI (International Rice Research Institute). Brown planthopper (BPH): threat to rice production in Asia. Los Baños (Philippines): International Rice Research Institute. 1979;369.
Velusamy R, Heinrichs EA. Electronic monitoring of feeding behaviour of Nilaparvata lugens (Homoptera: Delphacidae) on resistant and susceptible rice cultivars. Environ.Entomol. 1986;15: 678-682.
Khush GS. Disease and insect resistance in rice. Adv. Agron. 1977a;29:265-341.
Tang K, Zhao E, Sun X, Wan B, Qi H, Lu X. Production of transgenic rice homozygous lines with enhanced resistance to the rice brown planthopper (BPH). Acta Biotechnol. 2001b;21:117-128.
Khush GS. Rice breeding for disease and insect resistance at IRRI. Oryza. 1971;8:111-119.
Khush GS, Karim AR, Angeles ER. Genetics of resistance of rice cultivar ARC 10550 to Bangladesh brown planthopper (BPH) biotype. J. Genet. 1985;64:121-125.
Lakashminarayana A, Khush GS. New genes for resistance to the brown planthopper (BPH) in rice. Crop Sci. 1977;17:96-100.
Chen JW, Wang L, Pang XF, Pan H. Genetic analysis and fine mapping of a rice brown planthopper (BPH) (Nilaparvata lugens Stål) resistance gene bph19(t). Mol. Gen. Genomics. 2006;275:321-329.
Wang Y, Wang X, Yuan H, Chen R, Zhu L, He R, He G. Responses of two contrasting genotypes of rice to brown planthopper (BPH). Mol. Plant-Microbe Interact. 2008a; 21:122-132.
Jackson MT. Conservation of rice genetic resources: The role of the International Rice Genebank at IRRI. Plant Mol. Biol. 1997;35:61-67.
Li JB, Xia MY, Q HX, He GC, Wan BL, Zha ZP. Marker-assisted selection for brown planthopper (BPH) (Nilaparvata lugens Stål) resistance genes Bph14 and Bph15 in rice. Sci. Agric. Sin. 2006;39: 2132-2137.
Zhang F, Zhu L, He G. Differential gene expression in response to brown planthopper (BPH) feeding in rice. J. Plant Physiol. 2004;161:53-62.
Padmarathi G, Ram T, Ramesh K, Kondala Rao Y, Pasalu IC, Viraktamath BC. Genetics of white backed planthopper, Sogatella furcifera (Horvath) resistance in rice. SABRAO J. 2007;39:99-105.
Angeles ER, Khush GS, Heinrichs EA. Inheritance of resistance to planthoppers and leafhoppers in rice. In: Rice genetics. Manila (Philippines): International Rice Research Institute. 1986;537-549.
IRRI, Standard Evaluation System for Rice, Manila, Philippines: International Rice Research Institute; 1988.
The Rice Knowledge Bank.
Dellaporta SL, Wood T, Hicks TB. A plant DNA mini preparation: Version II. Plant Mol Bioi Rep. 1983;1:19-21.
Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C. Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of chromosome 12. Cereal Res. Commun. 2001;29:245-250.
Ikeda R, Kaneda C. Genetic analysis of resistance to BPH Nilaparvata lugens Stål in rice. Jpn. J. Breed. 1981;31:279- 285.
Panda N, Khush GS. Host plant resistance to insects. Wallingford (UK): CAB International. 1995;431.