PARASITIC AND PATHOGENIC EFFECTS OF Fuarium oxysporum f. sp. albedinis ISOLATES ON OKRA AND COLLARD PLANTS

Main Article Content

SMAIL ABDELLAOUI
AICHA EL AISSAMI
OMAR BENKHEMMAR
MOSTAPHA ARAHOU
AMINA OUAZZANI TOUHAMI
ALLAL DOUIRA

Abstract

Parasitic and pathogenic effects of four isolates of Fusarium oxysporum f. sp. albedinis, originating from the date palm, were tested on okra (Abelmoschus  esculentus) and collard (Brassica oleracea var. viridis) plants, two plant species largely associated with date palm in a Moroccan oasis. Inoculation of the plants was carried out by soaking their roots in a spore suspension (106 spores/ml) of the Fusarium oxysporum f. sp. albedinis isolates. The presence of the fungus in the inoculated plants stimulated the growth of the plants. The length of the okra plants inoculated with isolates 1, 2 and 4, varies between 33.33 and 52.1 cm compared to the control plants (28.5 cm). The number of leaves emitted from inoculated plants varies from 15.22 to 18.74 while the number of leaves emitted from control plants is 12.33. The fresh weight of the aerial part varies between 30.86 and 34.73 g and the root part varies between 30.86 and 34.73 g and between 48.1 and 50.8 g, respectively, those of the control plants are 41.2 and 49.1 g. Although isolate 3, induced stunting on okra plants, the length of the inoculated plants was 26 cm compared to the control plants with 28.5 cm. This reduction also affected the fresh weight of the aerial and the root part with26.36 g and 49.08 g, respectively; those of the controls are 30.96 and 48.1 g. All Fusarium  oxysporum f. sp. albedinis isolates tested have induced foliar symptoms in inoculated okra plants, with evidence of foliar alterations ranging from 0.38 (isolate 3) to 0.75 (isolate 1). Concerning inoculated plants of collard, isolates of Fusarium oxysporum f. sp. albedinis were also able to penetrate and colonize the upper levels of the plants, but the growth of these inoculated plants was not impeded, ranged from 11.16 to 11.96 cm with no difference as compared to the control plants (12 cm). The plants of collard inoculated with the studied isolates showed heterogeneous growth, some inoculated plants developed more than others and the average root mass of these plants (varies between 14.07 and 14.8 g) exceeds that of control plants (10.6 g). The parasitic specificity of  Fusarium oxysporum f. sp. albedinis  was also studied in this research.

Keywords:
Fusarium oxysporum f. sp. albedinis, date palm, parasitic effect, pathogenic effect, inoculation, okra, collard, symptoms, parasitic specificity.

Article Details

How to Cite
ABDELLAOUI, S., AISSAMI, A. E., BENKHEMMAR, O., ARAHOU, M., TOUHAMI, A. O., & DOUIRA, A. (2020). PARASITIC AND PATHOGENIC EFFECTS OF Fuarium oxysporum f. sp. albedinis ISOLATES ON OKRA AND COLLARD PLANTS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(5-6), 16–23. Retrieved from https://www.ikpresse.com/index.php/PCBMB/article/view/4951
Section
Original Research Article

References

Hamood HH, Mawlood EA. The effect of mechanical pollination on fruit set, yield and fruit characteristics of date palm (Phoenix dactylifera L.) Zahdi cultivar. Date Palm Journal. 1986;4(2):175-184.

Anonymous. MEDD. Ministry of the Environment and Sustainable Development. Direction General of the Environment and Quality of Life, Project development of a monography full of oases in Tunisia. Document 1. Identification and Characterization of Oasis in Tunisia. 2015;318.

Rhouma A. Synthesis report of the workshop' genetic diversity in oases. In Ferry M. (Ed.), Greiner D. (Ed.). The date palm in the oasis agriculture of Mediterranean countries. Zaragoza: CIHEAM. 1993;159-160.

Gómez Vives S, Ferry M. Biological control of the main pests in palm groves: State knowledge and research needs. Proceedings of the International Symposium on Sustainable Development of Oasis Systems from 08 to 10 March 2005, Erfoud, Morocco, B. Boulanouar & C. Kradi (Eds.). 2005;44.

CMI. Descriptions of pathogenic fungi and bacteria, Fusarium oxysporum f. sp. albedinis. CAB International, Wallingford (GB). 1992;1111.

CMI. Descriptions of pathogenic fungi and bacteria, Fusarium oxysporum. CAB International, Wallingford (GB). 1978;211.

EPPO/CABI. Aleurocanthus woglumi. In: Quarantine Pests for Europe. CAB International, Wallingford (GB). 2nd Edn. 1997;25-29.

Arai K, Yamamoto A. New Fusarium disease of Canary Island date palm in Japan. Bull. Fac. Agric. Kagoshima Univ. 1977;27:31-37.

Mercier S, Louvet J. Research on Fusarium wilt. X. Fusarium wilt of the palm of the Canaries. In: Ann. Phytopathol. 1973;5:203-211.

Sedra MH, Djerbi M. Comparative study of morphological characteristics and pathogenicity of two Fusarium oxysporum causing respectively the vascular wilt disease of date palm (Bayoud) and Canary Island palm. In: Proceedings of the Second Symposium on the Date Palm, Saudi Arabia. 1986;359-365.

Guillemat J, Montegut J. Contribution to the study of the fungal microflora of cultivated soils I, II. Arm. Epiphyt. 1958;9:27-54.

Armstrong GM, Armstrong JK. Formae speciales and races of Fusarium oxysporum. In: Nelson PE, Toussoun TA and Cook RJ (Eds) Fusarium: Diseases, Biology and Taxonomy. The Pennsylvania State University, University Park. 1981;391-399.

Snyder WC, Hansen HN. The species concept in Fusarium. Ann. J. Bot.1940;27:64-67.

Djerbi M, El Ghorfi A, El Idrissi Ammari MA. Study of the behavior of henna (Lawsonia inermis) and alfalfa Medicago sativa and some opposite palmaceous species of Fusarium oxysporum f. sp. albedinis, causative agent of bayoudh. Annals of the National Institute of Agronomic Research of Tunisia. 1985;58:1-11.

Djerbi M, Saaidi M, Sedra MH. A new fusarium wilt (bayoudh) -like disease on Canary Island palm Phoenix canariensis in Morocco. In: Proceedings of the Second Symposium on Date Palm, Saudi Arabia. 1986;375-381.

Henni J, Boisson, Geige JP. Variability of pathogenicity in Fusarium oxysporum f. sp. lycopersici. Phytopath. Medit. 1994;33:10-16.

Sghir F, Touati J, Chliyeh M, Ouazzani Touhami A, Filali-Maltouf A, Cherkaoui E, Moukhli A, Oukabli A, Benkirane R, Douira A. Variation in pathogenicity of Fusarium oxysporum f. sp. albedinis on two cultures associated with date palme of Moroccan oasis. World Journal Pharmaceutical and Life Science. 2016;2(3): 56-68.

Woo SL, Zoina A, Del Sorbo G, Lorito M, Nanni B, Scala F, Noveiello C. Characterization of Fusarium oxysporum f. sp. phaseoli by pathogenic races, VCGs, RFLPs, and RAPD. Phytopathol. 1996;86:966-972.

Douira A, Lahlou H. Variability of parasite specificity in Verticillium albo-atrum Reinke and Berthold, microsclerotic form. Crypt, Mycol.1989;10(1):19-32.

Goudou S. Cica. Les relations hôte-parasite dans le cas du couple tomate - Verticillium dahliae: Etudes histologiques. Montpellier. ORSTOM.29. Mem. DEA. Physiologie. USTL. Montpellier; 1988.

Gabriel M. Estimation of the aggressiveness of different Fusarium oxysporum f. sp. lycopersici (Schlecht). Mycopathologia. 1977; 62(l):61-64.

EL Aissami A. Contribution to the study of the variability of the pathogenic power of Verticillium dahliae. Moroccan Journal of Plant Protection. 2011;2: 11-17.

Armstrong GM, Hawkins BS, Benett CC. Cross inoculation with isolates of fusariam from cotton, tobacco and certain other plants subject to wilt. Phytopathol. 1942;32:685-698.

Bouhout D, Mallamaire A. Les principales maladies des plantes cultivées au Sénégal. Tomes 1 et 2. Grande Imprimerie africaine de Dakar; 1965.

Drame A. Etude des possibilités d’utilisation de la solarisation dans la lutte contre les maladies du sol. Thèse de Doctorat Ingénieur, Institut National Agronomique Paris Grignon (INAP- G). 1985;70.

Drame A. Pathogénie comparée de quelques souches de Fusarium oxysporum f. sp. vasinfectum Atk., agent de la fusariose du Gombo (Abelmoschus esculentus L.) au Sénégal; 2004.

Zehhar G, Ouazzani Touhami A, Badoc A, Douira A. Effet des Fusarium des eaux des rizières sur la germination et la croissance des plantules du riz. Bull. Soc. Pharmacol Bordeaux. 2006;145:7-18.

Beye I, Lafay JF. Verticillium wilt of tomato consequences of interactions between pathogen aggressiveness and resistance of the host at the population level. Agronomie Paris. 1988;8:435-440.

Selman IW, Buckley WR. Factors affecting the invasion of tomato roots by Verticillium albo-atrnm. Trans. Brit. Mycol. Soc. 1959;42(2):227-234.

Aube C, Sakston WE. Distribution and prevalence of Verticillium species producing substances with gibberelline-like Biological properties. Canad. J. Bot. 1965;43(11):1335-1342.

Chevaugeon J. Mode d’action des champignons parasites. Extrait du Bull. de la Soc. Bot. Fr. 1957;104:1-2.

Anonymous 2. The cards of exotic plants; 2009.
Available:http://gardenbreizh.org/module/gbdb/plante